By Bhowmick D., Davison A.C.
Read or Download A Laplace mixture model for identification of differential expression in microarray experiments (200 PDF
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Additional resources for A Laplace mixture model for identification of differential expression in microarray experiments (200
0 mV plotted in Fig. 4 were calculated from the volume pumped over 10-h intervals (see Sect. 2). To illustrate how the turbidostat system responds to a perturbation, algae were added abruptly to a steady-state culture, and D was then calculated at 10-h intervals before, during, and after the perturbation (Fig. 5A). 2 days, which resulted in a decrease in culture transmittance. D declined because the optical density was above the set point. Although the actual D of the culture was reduced to zero at the time of algal addition, the calculated D (from the number of pump events per lO-h interval) shows a positive value after the addition (Fig.
Thus, practical turbidostats are diluted at frequent intervals rather than continuously. The organisms, however, grow continuously. It is usually not difficult to operate the system with sufficient sensitivity so that the interval between dilutions is a small fraction of the doubling time of the population, effectively resulting in continuous operation. Turbidostats and chemostats are complimentary systems with regard to measurements of a population's response to its limiting nutrient and the dilution or per capita growth rate (Fig.
2 x 10- 8 gil om x 10- 8 gil Chu 10 (modified by Williams 1971; Boraas 1983). Other culture media for rotifers are given by King and Snell (1978) and Reed (1979). Trace elements are necessary, but must be used with caution. 1) is too concentrated for Keratella cochlearis. Therefore, only half of this concentration was used (Walz 1983a), but a steady state in a Stichococcus chemostat could not be achieved even with that concentration (Walz 1983b). Rotifer culture usually requires free vitamins. 1 gives the concentrations of the vitamin solution of Provasoli et al.